Biology grouping and drug resistance of Haemophilus influenzae from respiratory tract infection in children

Objective To investigate the distribution of biotype and drug resistance of Haemophilus influenzae(HI) from respiratory tract infection in children in Suzhou district,China.Methods Respiratory tract secretions for culture was obtained by vacuum suction and biology grouping was done for the isolation of HI.The beta-lactamase of HI was detected by nitrocefin and drug resistance was tested by Kirby-Bauer disc method.Results A total of 154 strains of HI was isolated,among which biotypes of Ⅱand Ⅲ were the two most common ones,and accounted for 81.2%.There was a significant season difference of the detection rate for biotypes of Ⅱ,Ⅲ and Ⅶ(P0.05).The beta-lactamase positive rate was 33.3% for biotypeⅠ,41.7% for biotypeⅡ,32.3% for biotype Ⅲ,35.7% for biotype Ⅶ and 25.0% for biotype Ⅷ.The resistance rate of HI to ampicillin was 35.7% and the muti-resistance rate of HI was 22.7%,mainly producing beta-lactamase of HI.Conclusion The biotypes of Ⅱ and Ⅲ are the main common types in Suzhou district.The beta-lactamase positive rate of biotypeⅡ is the highest one.The situation of drug resistence of HI has been serious day by day.