Influence of Survivin gene on biological behaviours of colorectal cancer HT29 cells

Purpose To explore the influence of Survivin gene on the proliferation and apoptosis of HT29 cells.Methods Survivin-targeted siRNA was transfeted into human colorectal cancer HT29 cells with Lip-2000 medium.Expression of survivin protein was detected by immunocytochemistry and Western blot.Cell proliferation was measured by MTT assay.Cell apoptosis was detected by flow cytometry(FCM).Results Immunocytochemical results indicated that Survivin had strongly positive expression in the normal control group,Lip-2000 control group and mismatch control group,and showed weak expression in Survivin-siRNA interference group.Western blot indicated that brightness of protein bands in Survivin-siRNA interference group was significantly lower than those in normal control group,Lip-2000 control group and mismatch control group.Compared with control group,the inhibitory rate of cell proliferation was significantly increased in Survivin-siRNA interference group compared with negative mismatch group(P0.05).Cell proliferation rates were significantly different between 24,48 and 72 h after transfection(P0.05).FCM showed that diploid peak appeared in Survivin-siRNA interference group and cell apoptosis rates of Survivin-siRNA interference group were obviously higher than those of empty control groups(P0.01).Conclusion Survivin gene regulates the proliferation of colorectal cancer cells and induces apoptosis,which may become a new target in gene therapy for colorectal cancer.