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Effects on mRNA expression of muscarinic acetylcholine receptors in aorta endothelium by carbachol and pilocarpine

Hai Wang

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Abstract

AIM: To study the modulation on mRNA expression of muscarinic acetylcholine receptors in bovine aorta endothelial cells by carbachol and pilocarpine. METHODS: Total RNA was extracted from the bovine aorta endothelial cells having been incubated with carbachol and pilocarpine at the dose of 10~(-4) mol·L~(-1) for 10hr. The expression of muscarinic acetylcholine receptor subtypes mRNA was measured by RT-PCR. RESULTS: The mRNA expression of five muscarinic acetylcholine receptors were all measured. These five genes were up-reg- ulated after incubated with carbachol and pilocarpine at the dose of 10~(-4)mol·L~(-1) for 10hr and there was no difference in them. CONCLUSION: Carbachol and pilocarpine has the same effect on genes expression of muscarinic acetylcholine receptor subtypes in aorta endothelial cells. Their effects on muscarinic acetylcholine receptor subtypes can not explain the influence to vascular ten-

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AIM: To study the modulation on mRNA expression of muscarinic acetylcholine receptors in bovine aorta endothelial cells by carbachol and pilocarpine. METHODS: Total RNA was extracted from the bovine aorta endothelial cells having been incubated with carbachol and pilocarpine at the dose of 10~(-4) mol·L~(-1) for 10hr. The expression of muscarinic acetylcholine receptor subtypes mRNA was measured by RT-PCR. RESULTS: The mRNA expression of five muscarinic acetylcholine receptors were all measured. These five genes were up-reg- ulated after incubated with carbachol and pilocarpine at the dose of 10~(-4)mol·L~(-1) for 10hr and there was no difference in them. CONCLUSION: Carbachol and pilocarpine has the same effect on genes expression of muscarinic acetylcholine receptor subtypes in aorta endothelial cells. Their effects on muscarinic acetylcholine receptor subtypes can not explain the influence to vascular ten-

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Available abstract

AIM: To study the modulation on mRNA expression of muscarinic acetylcholine receptors in bovine aorta endothelial cells by carbachol and pilocarpine. METHODS: Total RNA was extracted from the bovine aorta endothelial cells having been incubated with carbachol and pilocarpine at the dose of 10~(-4) mol·L~(-1) for 10hr. The expression of muscarinic acetylcholine receptor subtypes mRNA was measured by RT-PCR. RESULTS: The mRNA expression of five muscarinic acetylcholine receptors were all measured. These five genes were up-reg- ulated after incubated with carbachol and pilocarpine at the dose of 10~(-4)mol·L~(-1) for 10hr and there was no difference in them. CONCLUSION: Carbachol and pilocarpine has the same effect on genes expression of muscarinic acetylcholine receptor subtypes in aorta endothelial cells. Their effects on muscarinic acetylcholine receptor subtypes can not explain the influence to vascular ten-

Key concepts: Carbachol, Muscarinic acetylcholine receptor, Pilocarpine, Acetylcholine, Muscarinic acetylcholine receptor M2, Muscarinic acetylcholine receptor M3, Endocrinology, Muscarinic acetylcholine receptor M1

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Effects on mRNA expression of muscarinic acetylcholine receptors in aorta endothelium by carbachol and pilocarpine — Research Paper | ScholarLens