RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton

【Objective】 Identification of the differences of mitochondrial genomes between cytoplasmic male sterile(CMS) line and maintainer line of cotton could make a foundation for cloning CMS-associated gene in cotton.【Method】In this study,20 mitochondrial gene probes,atpA,atp6,atp9,ccmB,ccmC,ccmFN1,cob,coxI,coxII,coxIII,matR,nad1bc,nad2,nad4,nad5,nad6,nad7c,nad9,rpl5,and rrn18,were used to identify the differences between the P30B normal fertile(N-) and the P30A male sterile(S-) cytoplasm with Southern blot method.【Result】Among the 20 genes,atpA,atp9,ccmB,nad1bc,nad6,nad7c and rrn18 revealed restriction fragment length polymorphisms(RFLPs) between N-and S-cytoplasm.The differences in atpA locus were most obvious.【Conclusion】 atpA was an important mitochondrial genomic site that showed an obvious difference between CMS line and maintainer line of cotton.One rrn18 gene copy was deleted in the mitochondrial genome of CMS line.