The Inhibition of HBV Replication by Interferon-Induced MXA Protein in Vitro

Objective To investigate the antiviral activity of interferon-induced MXA protein against hepatitis B virus.Methods The HepG2 cells in MXA groups were cotransfected with pcDNA3.1-MXA and PU19-1.24HBV in a ratio of 1 ∶ 1 or 1 ∶ 2.The control groups were cotransfected with pcDNA3.1,or salmon DNA and PU19-1.24HBV.After 3 days,the HBsAg and HBeAg in the supernatant were measured by detection kit(Abbott).The extracellular and intracellular HBV DNA was detected by real-time PCR.MXA expression was detected by Western blot.Results MXA protein was expressed in MXA groups.When the cotransfection ratio is 1 ∶ 1,compared with the control groups,HBeAg was decreased by 27%,and the extracellular and intracellular HBV DNA were decreased by 1 and 0.6 logs,respectively.When the ratio is 2 ∶ 1,compared with the control groups,HBeAg was decreased by 66%,and the extracellular and intracellular HBVDNA were decreased by 1.9 and 1.7 logs,respectively.The MXA expression shows no marked difference between group cotransfected with the MXA vector and PU19-1.24HBV and the control group.Conclusion MXA protein could inhibit the replication of HBV in the HepG2 cells and it maybe not degrade while inhibiting the HBV production.