Comparison of the Umbilical Cord Mesenchymal Stem Cells Cultured by Serum-free Medium and Fetal Bovine Serum-contained Medium

To compare the differences of proliferation,biological characteristics,differentiation potential of serum-free and fetal bovine serum-contained medium cultured human umbilical cord-derived mesenchymal stem cells. Separated Wharton's jelly from human umbilical cord,cultured umbilical cord mesenchymal stem cells in serum-free medium and serum-supplied medium. Detected the cells' proliferation rate by MTT method and get the cell growth curve; observe the cell morphology by microscope; Use flow cytometer to identify surface markers CD73,CD90,CD105,CD14,CD34,CD45,CD79 a,and HLA-DR. We observe cells change of shape after cells cultured by Osteogenesis Differentiation and Adiposeness Differentiation medium. Use alkaline phosphatase and oil red O stai-ning methods to ensure cells' differentiation. The cell morphology,proliferation activity,surface markers and differentiation potential of human umbilical cord mesenchymal stem cells cultured in serum-free medium are similar with which cultured in FBS-supplied medium. The composition of the serum-free medium are animal origin free and the composition are known,so it's a better human umbilical cord mesenchymal stem cell culture media medium