Effect of Tanreqing Injection on Anti-Oxidant Activities of Acute Lung Injury in Rats

Objective:To study the effects of Tanreqing injection on anti-oxidant activities of acute lung injury(ALI)in rats.Methods:Fifty-six clean grade healthy male SD rats were randomly divided into three groups:normal group,model group and treatment group.Rats in the model group and treatment group were injected with lipopolysaccharide(LPS)into tail vein.Rats in treatment group were treated with Tanreqing injection after one hour of LPS injection.The observing intervals were respectively set in 2h,4h and 6h.At each observing interval,the ratio of neutrophiles(PMN%)in BALF were calculated by Wright-Giermsa staining,the pathological changes of lungs were observed,the wet/dry weight ratio(W/D)of lungs were examined and the changes of MPO、MDA、ET in lung tissues were detected.Data were analyzed with SPSS version 17.0 software.Results:At 2h,4h and 6h intervals,the pathological changes of lung tissues in model group were severe.PMN% in BALF,the lung W/D and MPO、MDA、ET in lung tissues were significantly higher in model group than those in normal group(P0.05 or P0.01).At each observing interval,compared with model group,the pathological changes of lung tissues,PMN% in BALF,the lung W/D and MPO、MDA、ET in lung tissues were significantly reduced in treatment group(P0.05 or P0.01).Conclusion:Tanreqing injection could provide partly protection in rats with ALI by anti-oxidant activities.