Inhibition effect and mechanisin of NS-398 on HepG2 cell proliferation

Objective To invesigate inhibition effect and mechanism of NS-398 on HepG2 cells.Methods HepG2 cells were treated with various concentrations(100,200,300,400μmol/L)of NS-398.MTT method was used to detect cell proliferatien inhibition rate.DNA fragmentation gel analysis was used to analyze the cell apoptosis.DNA ploidy cell percentage were examined by flow cytometry(FCM).The expression of VEGF was also examined by SP Immunohistochemistry and Western blot analysis.Results NS-398 in hibited HepG2 cells proliferration and induced apoptosis in a concentration-dependent manner.DNA ploidy analysis showed that S phase cell were significantly decerased and quiescent G1 phase was accumulated with NS-398 concentration increasing.The IC50 of 24 hours was 300 μmol/L.There was significant difference between the expression of VEGF in 200、300、400 μmol/L NS-398 groups and control groups.Conclusion NS-398 can inhibit the proliferation and induce apoptosis in HepG2 cells.The mechanism may be related with the accumulation of quiescent G1 phase and the inhibition of VEGF activity.