Genotyping study of 208 Mycobacterium tuberculosis clinical isolates from Guangxi with Spoligotyping

To study the genotypes of Mycobacterium tuberculosis(M.tuberculosis) strains isolated from Guangxi,China with spacer oligonucleotide typing(Spoligotyping) and evaluate the relationship between Beijing family and drug resistance.M.tuberculosis clinical strains isolated from the patients with tuberculosis(TB) in Guangxi,and tested the susceptibility of the isolates to 4 anti-TB drugs(INH,SRM,RFP,EMB) with the proportion method and did genotyping wiht Spoligotyping.The data were analyzed to clustering with BioNumerics software and statistics ananlysis with Chi-Square Tests.Clinical of 208 isolates of M.tuberculosis were collected in Guangxi.By means of Spoligotyping,these strains were typed into two groups which belong to Beijing family and Non-Beijing family,55.3%(115/208)and 44.7%(93/208)respectively;47 genotypes,in which there were 36 single types and the other 172 strains were including 11 clusters.In Beijing family strains 90.4%(104/115) is Typical Beijing family.There were more polymorphisms in Non-Beijing family strains in which 93 strains could be subdivided into 39 genotypes including 31 single genotypes.Comparison with the results of drug susceptibility of 205 strains in Beijing family strains there were 76.25%(76/113) sensitive strains,and 23.75%(37/113) drug resistant strains,whereas in Non-Beijing family strains,there were 75% sensitive strains while 25% drug resistant.There was no significant difference in statistics analyzed with Chi-Square Tests(χ2=2.9721,P0.05).There is high allelic diversity in Guangxi M.tuberculosis.And there might be no relationship between Beijing genotype and drug-resistance.