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2013•Journal of Qingdao University of Science and TechnologyRequires access

Preparation and Characterization of an ECL-Triplex Biosensor

Sujuan Ye

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Abstract

A novel ECL-triplex biosensor based on the ferrocene quenching of tris(2,2′-bipyridyl)ruthenium uminescence was developed.First the DNA tagged with tris(2,2′-bipyridyl)ruthenium was self-assembled onto the surface of gold electrodes.And then it hybrided with a specific sequence of DNA and ferrocene labeled DNA,in the presence of binding agent to form the structure of triplex DNA.The concentration of the synthesized of luminous markers was calibrated,and the probe solution was characterized,and the binding agent was choosed.Finally a viable triplex DNA biosensor was constructed.

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What this paper is about

A novel ECL-triplex biosensor based on the ferrocene quenching of tris(2,2′-bipyridyl)ruthenium uminescence was developed.First the DNA tagged with tris(2,2′-bipyridyl)ruthenium was self-assembled onto the surface of gold electrodes.And then it hybrided with a specific sequence of DNA and ferrocene labeled DNA,in the presence of binding agent to form the structure of triplex DNA.The concentration of the synthesized of luminous markers was calibrated,and the probe solution was characterized,and the binding agent was choosed.Finally a viable triplex DNA biosensor was constructed.

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Available abstract

A novel ECL-triplex biosensor based on the ferrocene quenching of tris(2,2′-bipyridyl)ruthenium uminescence was developed.First the DNA tagged with tris(2,2′-bipyridyl)ruthenium was self-assembled onto the surface of gold electrodes.And then it hybrided with a specific sequence of DNA and ferrocene labeled DNA,in the presence of binding agent to form the structure of triplex DNA.The concentration of the synthesized of luminous markers was calibrated,and the probe solution was characterized,and the binding agent was choosed.Finally a viable triplex DNA biosensor was constructed.

Key concepts: Ferrocene, Biosensor, Ruthenium, Tris, DNA, Combinatorial chemistry, Chemistry, Quenching (fluorescence)

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