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2005•Journal of Clinical StomatologyRequires access

Studies on optimization of mineralization condition of bone marrow stromal cells

Jin Ya

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Abstract

Objective:To investigate the biological character of bone marrow stromal cells in different mineralization condition .Method:The third passage BMSCs were induced by mineralization condition medium , BMP and the two combined. After 3 days, the proliferation and alkaline phosphatase level of cells were investigated,and collagen type Ⅰ were examined by immunohistochemistry stains.Result: Compared with control group, the proliferation of BMSCs in experimental group decreased, but ALP level increased and type I collagen was positive. Of the three experimental groups, the combined group significantly express the osteoblastic marker. Conclusion:BMSCs in the combined group can be induced to differentiare into osteoblasts in a shorter culture time.

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Objective:To investigate the biological character of bone marrow stromal cells in different mineralization condition .Method:The third passage BMSCs were induced by mineralization condition medium , BMP and the two combined. After 3 days, the proliferation and alkaline phosphatase level of cells were investigated,and collagen type Ⅰ were examined by immunohistochemistry stains.Result: Compared with control group, the proliferation of BMSCs in experimental group decreased, but ALP level increased and type I collagen was positive. Of the three experimental groups, the combined group significantly express the osteoblastic marker. Conclusion:BMSCs in the combined group can be induced to differentiare into osteoblasts in a shorter culture time.

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Available abstract

Objective:To investigate the biological character of bone marrow stromal cells in different mineralization condition .Method:The third passage BMSCs were induced by mineralization condition medium , BMP and the two combined. After 3 days, the proliferation and alkaline phosphatase level of cells were investigated,and collagen type Ⅰ were examined by immunohistochemistry stains.Result: Compared with control group, the proliferation of BMSCs in experimental group decreased, but ALP level increased and type I collagen was positive. Of the three experimental groups, the combined group significantly express the osteoblastic marker. Conclusion:BMSCs in the combined group can be induced to differentiare into osteoblasts in a shorter culture time.

Key concepts: Stromal cell, Mineralization (soil science), Alkaline phosphatase, Bone marrow, Immunohistochemistry, Chemistry, Type I collagen, Pathology

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