Genetic Diversity of Angelicae dahuricae Accessions from Sichuan Province by Inter-simple Sequence Repeat(ISSR)

Inter-simple sequence repeat(ISSR) method was applied to detect genetic variation of Angelicae dahuricae accessions from Suining,Sichuan province.Fifteen primers were selected from 35 ISSR primers,and 99 DNA fragments were amplified from 16 samples,38 fragments among them were polymorphic(percentage of polymorphic bands was 38%).The genetic similarity among all accessions ranged from 0.821 to 0.994,which showed little genetic difference among the tested accessions.The average of genetic similarity coefficient between Angelica dahurica var.formosana and Angelica dahurica var.formosana from Sichuan province was larger than the data among Angelica dahurica var.formosana from Sichuan province.UPGMA cluster based on genetic similarity divided the accessions into two groups,one group were all from Suining,and the other were from other regions,including two samples from the south of Fu river in Suining.This showed that some relation existed between the results of UPGMA and the geographical distribution of the samples.