Influence of hyperbaric oxygen therapy on rats neural stem cells proliferation after hypoxic-ischemic brain damage

Objective To investigate the influences of hyperbaric oxygen(HBO) and rat brain extracts on proliferation of rats neural stem cells in vitro.Methods Rats NSCs were randomly divided into five groups respectively according to the treatments as follows: blank control group(CON group): without any treatment;NC+N group: incubated with normal brain tissue extract conditioned cultures;NC+HIBD group: incubated with HIBD brain tissue extract conditioned cultures;NC+HIBD+HBO group: incubated with HIBD brain tissue extract conditioned cultures 1 h then treated with HBO;NC+N+HBO group: incubated with normal brain tissue extract conditioned cultures 1 h,then treated with HBO.Immunocytochemical staining was performed simultaneously on the 5 groups of NSCs.NSE-positive NSCs,O4-positive NSCs and GFAP-positive NSCs were counted for percentage of the positive cells′.Results Compared with CON,NC+N+HBO group,NC+N group the NC+ of HIBD percentage of cells which differentiated into neurons and oligodendrocytes increased significantly(P0.05),which HIBD group increased the most.On this basis,HBO treatment was given,differentiation of NSCs to neuronal would be further increased,there was a corresponding increase in the differentiation of oligodendrocytes;compared with CON,NC+N group,NC+HIBD group,NC+ of HIBD+HBO group differentiation,the percentage of astrocytes significantly reduced(P0.05).Conclusion HBO could promote ncNSCs differentiate into neuronal or oligodendrocyte,and inhibit ncNSCs differentiate into astrocytes.