Construction of IGF-1 Recombinant Baculovirus Expressive Vector and Its Expression in sf9 Cells

IGF-1 gene was amplified using PCR and cloned into pFastHTA with the EcoRⅠ and SalⅠ sites. The constructed pFast/IGF-1 was transformed into Escherichia coli DH10Bac. After screening with kanamycin, tetracycline, gentamicin and X-gal/IPTG, the recombinant baculovirus expressive vector pBacmid-Fa-IGF-1 was obtained. Then the sf9 cells were transfected using the recombinant pBacmid-Fa-IGF-1. After packing in the sf9 cells, recombinant viruses were obtained, which could be observed under electron microscope. A band of protein of about 13.0 kD was identified using Western-blot, which demonstrated that the IGF-1 was successfully expressed in the sf9 cells. The cell growth promotion rate was 24.4% measured using MTT method.