Inhibitory effect of arsenic trioxide on proliferation and expression of collagen of 3T3 fibroblast induced by recombinant interleukin 13

Objective To observe the effect of arsenic trioxide(As2O3)on proliferation and expression of collagen type I of 3T3 fibroblasts induced by recombinant interleukin 13(rIL-13). Methods 3T3 fibroblasts were cultured in vitro,which were divided into four groups:DMEM control group,rIL-13(80 ng/mL)group and rIL-13 with various concentrations of As2O3(2 μmol/L and 4 μmol/L)group.MTT assay was carried out to study cell proliferation;collagen type I(CoI I)was determined with Western blot. Results As2O3 significantly inhibited the proliferation of 3T3 fibroblasts induced by rIL-13 with a dose dependent manner(P0.01).The expression of collagen type I could be detected in all groups,Which in rIL-13(80 ng/mL)group was the strongest among four groups.But the expression of collagen type I in rIL-13 with different concentrations of As2O3(2 μmol/L and 4 μmol/L)groups was significantly lower than that of rIL-13 group(P0.01),and there was no significant difference between 4 μmol/L As2O3 + rIL-13 group and 2 μmol/L As2O3 + rIL-13 group(P0.05). Conclusion As2O3 may play an important role in inhibiting the proliferation and expression of collagen type I induced by interleukin-13.