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Identification of Nocardia farcinica isolated from clinical strains

Wan Kang-lin

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Abstract

To identify the species of Nocardia(N.) clinical isolates by DNA sequencing,BJ7 isolated from sputum of a patient suspected with tuberculosis,N.farcinica AY756551 and Mycobacterium tuberculosis H37Rv were used in this study.The identification on the strain was done based on culturing with L-J,PNB/TCH media and molecular biology methods.The special fragments of rpoB and 16S rDNA gene were amplified by PCR.The PCR amplification products of rpoB and 16S rDNA genes were sequenced and the DNA sequences were blasted on NCBI.The results of morphological characteristics and PNB/TCH medium showed that BJ7 was Non-tuberculosis Mycobacterium(NTM).The DNA homology of rpoB and 16S rDNA genes of BJ7 compared with that of AY756551 were 99% and 100% respectively.The results showed that the clinical isolate BJ7 was N.farcinica.It shows that sequencing analysis is a rapid and reliable method for Nocardia.spp species identification.

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What this paper is about

To identify the species of Nocardia(N.) clinical isolates by DNA sequencing,BJ7 isolated from sputum of a patient suspected with tuberculosis,N.farcinica AY756551 and Mycobacterium tuberculosis H37Rv were used in this study.The identification on the strain was done based on culturing with L-J,PNB/TCH media and molecular biology methods.The special fragments of rpoB and 16S rDNA gene were amplified by PCR.The PCR amplification products of rpoB and 16S rDNA genes were sequenced and the DNA sequences were blasted on NCBI.The results of morphological characteristics and PNB/TCH medium showed that BJ7 was Non-tuberculosis Mycobacterium(NTM).The DNA homology of rpoB and 16S rDNA genes of BJ7 compared with that of AY756551 were 99% and 100% respectively.The results showed that the clinical isolate BJ7 was N.farcinica.It shows that sequencing analysis is a rapid and reliable method for Nocardia.spp species identification.

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Available abstract

To identify the species of Nocardia(N.) clinical isolates by DNA sequencing,BJ7 isolated from sputum of a patient suspected with tuberculosis,N.farcinica AY756551 and Mycobacterium tuberculosis H37Rv were used in this study.The identification on the strain was done based on culturing with L-J,PNB/TCH media and molecular biology methods.The special fragments of rpoB and 16S rDNA gene were amplified by PCR.The PCR amplification products of rpoB and 16S rDNA genes were sequenced and the DNA sequences were blasted on NCBI.The results of morphological characteristics and PNB/TCH medium showed that BJ7 was Non-tuberculosis Mycobacterium(NTM).The DNA homology of rpoB and 16S rDNA genes of BJ7 compared with that of AY756551 were 99% and 100% respectively.The results showed that the clinical isolate BJ7 was N.farcinica.It shows that sequencing analysis is a rapid and reliable method for Nocardia.spp species identification.

Key concepts: rpoB, Biology, 16S ribosomal RNA, Nocardia, Microbiology, Polymerase chain reaction, Mycobacterium tuberculosis complex, Mycobacterium tuberculosis

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