2008•Journal of Jiangsu UniversityRequires access
Effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats
Kan Chun-ting
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Abstract
Objective: To investigate the effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats.Methods: Diabetic model was established by a single injection of streptozotocin(STZ),and diabetic rats were randomly divided in to 2 groups: untreated diabetes mellitus group(DM group) and rosiglitazone-treated diabetes mellitus group(DR group).In addition,the normal rats served as a normal control group(NC group).Eight weeks later,the excretion of urine protein,blood glucose,serum lipid profile,serum urea nitrogen and serum creatinine were detected.The expression of nephrin was detected by immunohistochemistry and real-time Q-PCR. Results: Compared with NC group,the excretion of urine protein,serum urea nitrogen and serum creatinine in DM group were significantly increased,and the expression of nephrin was significantly down-regulated.Compared with DM group,the excretion of urine protein,serum urea nitrogen and serum creatinine in DR group were significantly decreased,and the expression of nephrin was significantly up-regulated.Blood glucose and serum lipid profile were significantly changed in DM group compared with NC group,while they had no significant difference between DM group and DR group. Conclusion: Rosiglitazone may up-regulate the expression of nephrin in the kidney of diabetic rats to produce direct nephroprotective effects independent of glucose and lipid metabolism.