ScholarLens Back to search
2003•Journal of Anhui Normal UniversityRequires access

IN VITRO PROPAGATION OF DWARF CHERRY ROOTSTOCK GISELA

Huang Wen

Open publisher page 0 citations

Abstract

Stem explants and shoot tips obtained from a mature tree of cherry rootstock, Gisela5 and Gisela6(Prunus cerasus L.×P. canescens L.), were successfully established in vitro, and they were easing micro propagated on MS medium. As the nitrogen concentration in MS medium increase the shoot number increase too. The modified MS medium containing 3800mg/L potassium nitrate, 2745mg/L ammonium nitrate, 0.5mg/L benzyladenine and 0.1mg/L indolebutyric acid was optimal to form multiple shoot. After 10 times successive subcultures, 7-8 shoots per explant were produced. Shoot segment developed adventitious roots within 20 days in 1/2 strength MS medium containing 0.3mg/L indolebutyric acid. Eighty percent of the shoots rooted were successfully transplanted into greenhouse. 

About this research paper

What this paper is about

Stem explants and shoot tips obtained from a mature tree of cherry rootstock, Gisela5 and Gisela6(Prunus cerasus L.×P. canescens L.), were successfully established in vitro, and they were easing micro propagated on MS medium. As the nitrogen concentration in MS medium increase the shoot number increase too. The modified MS medium containing 3800mg/L potassium nitrate, 2745mg/L ammonium nitrate, 0.5mg/L benzyladenine and 0.1mg/L indolebutyric acid was optimal to form multiple shoot. After 10 times successive subcultures, 7-8 shoots per explant were produced. Shoot segment developed adventitious roots within 20 days in 1/2 strength MS medium containing 0.3mg/L indolebutyric acid. Eighty percent of the shoots rooted were successfully transplanted into greenhouse. 

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Stem explants and shoot tips obtained from a mature tree of cherry rootstock, Gisela5 and Gisela6(Prunus cerasus L.×P. canescens L.), were successfully established in vitro, and they were easing micro propagated on MS medium. As the nitrogen concentration in MS medium increase the shoot number increase too. The modified MS medium containing 3800mg/L potassium nitrate, 2745mg/L ammonium nitrate, 0.5mg/L benzyladenine and 0.1mg/L indolebutyric acid was optimal to form multiple shoot. After 10 times successive subcultures, 7-8 shoots per explant were produced. Shoot segment developed adventitious roots within 20 days in 1/2 strength MS medium containing 0.3mg/L indolebutyric acid. Eighty percent of the shoots rooted were successfully transplanted into greenhouse. 

Key concepts: Shoot, Rootstock, Explant culture, Murashige and Skoog medium, Prunus cerasus, Ammonium, Botany, Prunus

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
IN VITRO PROPAGATION OF DWARF CHERRY ROOTSTOCK GISELA — Research Paper | ScholarLens