Isolation and Identification of LA Strain of Pseudorabies Virus

Several virus strains have been isolated from the samples of brains and visceral organs of death piglets from some farms in Shandong province and one of them was identified.The virus was continually inoculated 5 generations onto rabbit kidney cell and chicken embryonic fibroblast cell(CEF) and induced typical cytopathic effect(CPE).The typical CPE could be seen when culturing the virus on RK 13 cell,PK15 cell,Vero cell,BHK 21 cell and 143 TK - cell and the TCID 50 of the isolated virus was 10 -6 52 /0 1 mL and the typical Pseudorabies virus particles could be found under electronmicroscope.The isolated virus was sensitive to chloroform and ethylether and inactivated for 30 minutes at 56℃,and could be neutralized by the positive serum to Pseudorabies virus.The typical clinical syndrome could be observed when four rabbits and 10 mice were injected with the isolate.The sequence of 262 base pair lying in gD gene could be amplified with the DNA extracted from the isolate and a set of oligonucleotide primers.All of the evidences indicated that the isolated virus was Pseudorabies virus(PRV) and was named as LA strain of Pseudorabies virus.