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Expression and significance of urokinase type plasminogen activator and its receptor in gastric cancer tissue by northern blot hybridization

Yang Chun

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Abstract

Objective:To study the expression of urokinase type plasminogen activator (uPA) and its receptor (uPAR) mRNA in gastric cancer tissues and the relationship with gastric cancer cell invasion and metastasis. Methods:uPA and uPA expression levels have been detected by cDNA mRNA northern blot hybridization in 20 gastric cancers and their surrounding tissues. Results:Of 20 gastric cancer cases, there were 13 and 15 had higher uPA and uPAR mRNA expressions than their surrounding tissues respectively ( P 0 01). Both uPA and uPAR mRNA expressions for 12 out of 20 were higher than that of surroundings. The uPA and uPAR mRNA expression levels in 11 gastric cancer tissues with lymph node metastases were obviously higher than those without lymph node metastases. Conclusion:The expression level of uPA and uPAR mRNA increase in gastric cancer tissues and obviously relates with gastric cancer cell invasion and metatasis.

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Objective:To study the expression of urokinase type plasminogen activator (uPA) and its receptor (uPAR) mRNA in gastric cancer tissues and the relationship with gastric cancer cell invasion and metastasis. Methods:uPA and uPA expression levels have been detected by cDNA mRNA northern blot hybridization in 20 gastric cancers and their surrounding tissues. Results:Of 20 gastric cancer cases, there were 13 and 15 had higher uPA and uPAR mRNA expressions than their surrounding tissues respectively ( P 0 01). Both uPA and uPAR mRNA expressions for 12 out of 20 were higher than that of surroundings. The uPA and uPAR mRNA expression levels in 11 gastric cancer tissues with lymph node metastases were obviously higher than those without lymph node metastases. Conclusion:The expression level of uPA and uPAR mRNA increase in gastric cancer tissues and obviously relates with gastric cancer cell invasion and metatasis.

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Available abstract

Objective:To study the expression of urokinase type plasminogen activator (uPA) and its receptor (uPAR) mRNA in gastric cancer tissues and the relationship with gastric cancer cell invasion and metastasis. Methods:uPA and uPA expression levels have been detected by cDNA mRNA northern blot hybridization in 20 gastric cancers and their surrounding tissues. Results:Of 20 gastric cancer cases, there were 13 and 15 had higher uPA and uPAR mRNA expressions than their surrounding tissues respectively ( P 0 01). Both uPA and uPAR mRNA expressions for 12 out of 20 were higher than that of surroundings. The uPA and uPAR mRNA expression levels in 11 gastric cancer tissues with lymph node metastases were obviously higher than those without lymph node metastases. Conclusion:The expression level of uPA and uPAR mRNA increase in gastric cancer tissues and obviously relates with gastric cancer cell invasion and metatasis.

Key concepts: Urokinase receptor, Northern blot, Messenger RNA, Cancer, Plasminogen activator, In situ hybridization, Metastasis, Cancer research

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Expression and significance of urokinase type plasminogen activator and its receptor in gastric cancer tissue by northern blot hybridization — Research Paper | ScholarLens