Effect of glucose on human umbilical vein endothelial cells activity and proliferation

Objective To investigate the effect of glucose on human umbilical vein endothelial cells(HUVECs).Methods HUVECs were cultured in vitro with endothelial cell medium.Different dose(10,20,30,40,50 mmol/L) of glucose were added to the HUVECs.Effect of glucose on the HUVECs activity and proliferation were determined by NO content,eNOS activity and cell apoptosis analysis.Results Glucose exhibited dose-dependent ihibitory effect on the HUVECs cell proliferative activity.Glucose could inhibit the effect of eNOs activity and the ability of produce NO on the HUVECs,also exhibited dose-dependence.Conclusions Glucose inhibited the activity and the ability of HUVECs,and the mechanism might be related to up-regulating cell apoptosis and ROS.