Study on the interaction between Taxol and Bovine Serum Albumin

The interaction between the Taxol and Bovine Serum Albumin(BSA) has been investigated by the ways of Fluorescence spectroscopy and Uv-vis absorption spectrometry.Under biological condition(make sure pH =7.4 by Tris-HCl buffer solution),with 286 nm as excitation wavelength,in the range of 288～500 nm,We determine the fluorescence spectroscopy of BSA.BSA has strong fluorescence effect and fluorescence quenching by Taxol.The results show the type f fluorescence quenching is static quenching.To measure the binding constants under the different temperatures,the results show that there is the strong binding effect between Taxol and BSA.