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2004•Zhongguo shouyi zazhiRequires access

Cloning and sequence analysis of interleukin-2 gene from the lymphocyte of tibetan pig

Rong Gao, Li Hui, Wu Mei, Wang Lihuan, Xiaotao Ding, Ping Li, Bo Li, Qing Yu, Shigui Liu

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Abstract

The interleukin-2 cDNA of Tibet Pig was amplified by RT-PCR from total RNA extracted from blood lymphocytes,which were cultivated and stimulated with 10 μg/ml ConA in vitro for 70 hours.Then the amplified cDNA was cloned into pMD-T vector and named as TPIL-2.The nucletide acid sequence of cloned Tibet pig interleukin-2 cDNA was determined.The length of the TPIL-2 was 503 bp(ORF was 465 bp),encoding a peptide of 154 amino acids whose molecular is 17.4 kd and pI is 5.38.By blasting the homologous sequences in GenBank databases,the sequence of Tibet pig interleukin-2 gene from lymphocyte is identical to the interleukin-2 genes previously cloned from spleen cell and fibroblast cell of other porcines.

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What this paper is about

The interleukin-2 cDNA of Tibet Pig was amplified by RT-PCR from total RNA extracted from blood lymphocytes,which were cultivated and stimulated with 10 μg/ml ConA in vitro for 70 hours.Then the amplified cDNA was cloned into pMD-T vector and named as TPIL-2.The nucletide acid sequence of cloned Tibet pig interleukin-2 cDNA was determined.The length of the TPIL-2 was 503 bp(ORF was 465 bp),encoding a peptide of 154 amino acids whose molecular is 17.4 kd and pI is 5.38.By blasting the homologous sequences in GenBank databases,the sequence of Tibet pig interleukin-2 gene from lymphocyte is identical to the interleukin-2 genes previously cloned from spleen cell and fibroblast cell of other porcines.

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Available abstract

The interleukin-2 cDNA of Tibet Pig was amplified by RT-PCR from total RNA extracted from blood lymphocytes,which were cultivated and stimulated with 10 μg/ml ConA in vitro for 70 hours.Then the amplified cDNA was cloned into pMD-T vector and named as TPIL-2.The nucletide acid sequence of cloned Tibet pig interleukin-2 cDNA was determined.The length of the TPIL-2 was 503 bp(ORF was 465 bp),encoding a peptide of 154 amino acids whose molecular is 17.4 kd and pI is 5.38.By blasting the homologous sequences in GenBank databases,the sequence of Tibet pig interleukin-2 gene from lymphocyte is identical to the interleukin-2 genes previously cloned from spleen cell and fibroblast cell of other porcines.

Key concepts: Complementary DNA, Molecular biology, GenBank, Biology, Gene, Cloning (programming), Sequence analysis, Molecular cloning

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Cloning and sequence analysis of interleukin-2 gene from the lymphocyte of tibetan pig — Research Paper | ScholarLens