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2011•Chinese Journal of Health Laboratory TechnologyRequires access

Establishment of multiplex real-time PCR assay for detection of Vibrio cholerae,Vibrio parahaemolyticus and Vibrio vulnificus

Jiang YingHui

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Abstract

Objective:Rapid detection of Vibrio cholerae;Vibrio parahaemolyticus and Vibrio vulnificus were established by multiplex real-time PCR assay.Methods: Seventeen test strains such as Vibrio alginolyticus was tested by specific detection.The different grades of standard strain were obtained by diluting and sensitivity was detected.Results: Compared with conventional method,the multiplex real-time PCR assay was specific and sensitively.Conclusion: The multiplex real-time PCR assay was rapid and accurate,with the detection limit of 280 cfu/ml for Vibrio cholerae,100 cfu/ml for Vibrio parahaemolyticus and 450 cfu/ml for Vibrio vulnificus.

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What this paper is about

Objective:Rapid detection of Vibrio cholerae;Vibrio parahaemolyticus and Vibrio vulnificus were established by multiplex real-time PCR assay.Methods: Seventeen test strains such as Vibrio alginolyticus was tested by specific detection.The different grades of standard strain were obtained by diluting and sensitivity was detected.Results: Compared with conventional method,the multiplex real-time PCR assay was specific and sensitively.Conclusion: The multiplex real-time PCR assay was rapid and accurate,with the detection limit of 280 cfu/ml for Vibrio cholerae,100 cfu/ml for Vibrio parahaemolyticus and 450 cfu/ml for Vibrio vulnificus.

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Available abstract

Objective:Rapid detection of Vibrio cholerae;Vibrio parahaemolyticus and Vibrio vulnificus were established by multiplex real-time PCR assay.Methods: Seventeen test strains such as Vibrio alginolyticus was tested by specific detection.The different grades of standard strain were obtained by diluting and sensitivity was detected.Results: Compared with conventional method,the multiplex real-time PCR assay was specific and sensitively.Conclusion: The multiplex real-time PCR assay was rapid and accurate,with the detection limit of 280 cfu/ml for Vibrio cholerae,100 cfu/ml for Vibrio parahaemolyticus and 450 cfu/ml for Vibrio vulnificus.

Key concepts: Vibrio vulnificus, Vibrio parahaemolyticus, Vibrio cholerae, Microbiology, Vibrio alginolyticus, Multiplex polymerase chain reaction, Vibrio, Multiplex

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Establishment of multiplex real-time PCR assay for detection of Vibrio cholerae,Vibrio parahaemolyticus and Vibrio vulnificus — Research Paper | ScholarLens