Chiral separation of chiral compounds on cellulose chiral column by HPLC

The chiral separations of three chiral compounds were directly performed on polysaccharide-based chiral OJ-H.The influence of the mobile phase modifier and the structures and types of alcohol on the chiral separation were investigated.Results indicated that the chiral separation depends strongly upon hydrogen bonding and the π-π interaction between the analyte and CSP molecules.Moreover,the carbon number of the alcohol in the mobile phase also affects the retention and resolution of the chiral compound.