2003Unpublished venueRequires access

Proliferative properties in vitro of adult rat neural stem cells from different brain regions

Ying Du

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Abstract

Aim: To investigate the distribution, conditions of incubation in uitro, and proliferative properties of adult rat neural stem cells. Methods:The cells isolated from striatuin,ventricular and subventricular zones of adult rats were cultured with serum-free medium and identified with immunocytochemistry, and its growth rules were observed at the same time. ResultS; The cells were able to proliferate to form neurospheres, its nestin immunocytochemical stain were positive, and its differentiated progeny expressed NSE and GFAP respectively. Conclusion ; The cells from specific zones have the characteristics of neural stem cell.

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Aim: To investigate the distribution, conditions of incubation in uitro, and proliferative properties of adult rat neural stem cells. Methods:The cells isolated from striatuin,ventricular and subventricular zones of adult rats were cultured with serum-free medium and identified with immunocytochemistry, and its growth rules were observed at the same time. ResultS; The cells were able to proliferate to form neurospheres, its nestin immunocytochemical stain were positive, and its differentiated progeny expressed NSE and GFAP respectively. Conclusion ; The cells from specific zones have the characteristics of neural stem cell.

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Available abstract

Aim: To investigate the distribution, conditions of incubation in uitro, and proliferative properties of adult rat neural stem cells. Methods:The cells isolated from striatuin,ventricular and subventricular zones of adult rats were cultured with serum-free medium and identified with immunocytochemistry, and its growth rules were observed at the same time. ResultS; The cells were able to proliferate to form neurospheres, its nestin immunocytochemical stain were positive, and its differentiated progeny expressed NSE and GFAP respectively. Conclusion ; The cells from specific zones have the characteristics of neural stem cell.

Key concepts: Neurosphere, Nestin, Neural stem cell, Subventricular zone, Immunocytochemistry, Stem cell, In vitro, Neuroepithelial cell

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