hTERT gene promoter modulated constrution of expressionvector of FADD

Objective To construct FADD expression vector modulated by human telomerase reserve transcriptase gene promoter.Methods Genomic fragment of hTERT promoter and FADD(Fas-associated death domain protein)were amplified by RT-PCR and inserted into the vector with GFP genomic fragment.The recombinant plasmid was identified by PCR and restriction endonuclease.The genomic fragment of hTERT promoter and FADD were determined and analyzed by DNA sequencing.Results Sequencing and restriction endonuclease digestion demonstrated that the fragment was cloned and included human telomerase reserve transcriptase gene promoter sequence.Conclusion The hTERT gene promoter has the tumor specificity,suggesting that the specific expression vector modulated by hTERT gene promoter may be a novel and promising approach to the tumor treatment.