2008•Yanke xinjinzhanRequires access
Curcumin on expression of VEGF and number of PMN in rabbit corneal neovascularization model
Wu Hai
Open publisher page 0 citations
Abstract
Objective To study the effect of curcumin on the number of polymorphonuclear leukocyte(PMN) and the expression of vascular endothelial growth factor (VEGF) in rabbit corneal neovascularization (CNV) model.Methods Forty normal New Zealand rabbits were divided into normal control group as Group A without any treatment(5 rabbits) and experimental groups with alkali burn for CNV models(35 rabbits),the right eye of which was not treated with curcumin as experimental control group(Group B),and the left eye with use of curcumin as experimental treatment group(Group C).The state of CNV was observed with slit lamp photography,the expressions of VEGF before and after using curcumin were detected by immunohistochemical staining,and the numbers of PMN were counted.All data were statistically analyzed.Results The expression of PMN and VEGF in Group A was negative or just weak at basement membrane of epithelium;But there was strong expression mainly at the region of CNV formation and all layers of corneal epithelium mainly in Group B.Compared with Group B,CNV was obviously less,and the expression of VEGF and PMN were weak at the region of CNV in corneal stroma in Group C.Conclusion Curcumin can effectively inhibit the growth of CNV induced by alkali burn in rabbit cornea by reducing the expression of VEGF and the number of PMN in CNV.