Inhibitory effects of RNAi targeting Survivin combined X-rays irradiation on grafting tumour of lung adenocarcinoma

Objective To approach the inhibitory effects of RNAi targeting Survivin combined X-rays irradiation on grafting tumour of lung adenocarcinoma and realative mechanism with RNAi,RT-PCR and Westren blot.Methods To design one pair RNA interference sequence targeting Survivin gene according to cDNA sequence showed in GeneBank,to construct the shRNA eukaryotic expressing vector pGenesil-2-Survivin.Normal group,pGenesil-2Survivin group,5 Gy irradiation group and pGenesil2-Survivin+5 Gy irradiation group were divided.Results RT-PCR and Western blot results showed that 5 Gy X-rays could not change Survivin mRNA and protein expressions obviously,but pGenesil-2-Survivin plasmid or pGenesil-2-Survivin plasmid adding 5 Gy X-ray irradiation could inhibit Survivin mRNA and protein expressions obviously,compared to normal group,it had statistical significance(P0.001);after 5 Gy X-rays irradiation,tumour growth was inhibited,but pGenesil-2-Survivin plasmid inhibitory effects was more obvious,and 5 Gy X-rays irradiation could enhanced the effect.Conclusion RNAi targeting Survivin could inhibit obviously the growth of nude mouse grafting tumour of lung adenocarcinoma,and combination with 5 Gy X-rays irradiation could enhance the inhibitory effects,these related to Survivin mRNA and protein expression.