Ultrasonographic evaluating the effect of implanted autologous bone marrow mesenchymal stem cells to promote angiogenesis in rabbit ischemic limbs:experimental study

Objective To investigate the significance and effect of ultrasonic diagnosis on the autologous bone marrow mesenchymal stem cells(MSC)in angiogenesis.Methods Twenty-four New Zealand rabbits were divided into experiment group(12)and the control group(12).Then rabbit bone marrow MSCs from experiment group were isolated,caltured and marked with Brdu.After ischemic hind limb animal model on all rabbits was set up,autologous bone marrow MSCs were directly injected into the ischemic hind limb muscles in experiment group while same volume normal saline was used in the control group.Two weeks after the implantation of autologous bone marrow MSCs,2D and color Doppler flow imaging(CDFI)detection were used in rabbit femoral artery of the two groups to observe the inner diameter of the blood vessel,the peak velocity and the acceleration time.The disposition of transplaned cells and the state of angiogenesis in ischemic muscles were assessed using immunofluorescence staining.Results The results of 2D and Doppler ultrasound detection showed the inner diameter of the blood vessel and the peak velocity of the blood current in experiment group obviously higher than that of the control group,and the acceleration time was obviously smaller than that of the control group P0.01.The immunofluorescence staining showed there were transplanted cells existed in transplanted portion and state of angiogenesis was supurior obviously than that of the control.Conclusions Bone marrow MSCs had the effect to promote angiogenesis.Implantation of autologous bone marrow MSCs was a simple and efficient therapeutic method for the ischemia hind limb.Using high-frequency ultrasound to detect femoral artery may provide a practical and useful method to evaluate the effect on implanted autologous bone marrow mesenchymal stem cells.