Effects of S1P5 on adhesion of human esophageal cancer cell line Eca109

Objective To investigate the effect of S1P5 on adhesion of human esophageal squamous cell carcinoma(ESCC) cell line Eca109.Methods Human S1P5 gene was cloned from normal esophageal mucosal epithelium.S1P5-EGFP vector was constructed and transfected into Eca109 cells.The S1P5 expressing Eca109 cell was designated S1P5-EGFP/Eca109.With Control-EGFP vector transfecting Eca109 cell as control,cell morphology and adhesion were analyzed by fluorescence microscopy and cell adhesion assay,respectively.Results S1P5-EGFP and Control-EGFP vectors,and their corresponding stably-transfected Eca109 cells were successfully constructed.S1P5 overexpressing Eca109 cells displayed spindle cell morphology and S1P5 was expressed on the plasma membrane.The cell adhesion of S1P5-transfected Eca109 cells was lower than control vector-transfected cells with or without sphingosine 1-phosphate(P0.05).Conclusion The data demonstrate that S1P5 constitutively inhibits Eca109 cell adhesion.Esophageal cancer cells may down-regulate the expression of S1P5 to promote adhesion.