Experimental Study on Ganle Granule in Rats Counteracting Hepatic Fibrosis Induced by Carbon Tetrachloride

Objective To investigate the inhibitory effect and possible mechanism of Ganle Granule(GG)on hepatic fibrosis induced by carbon tetrachloride(CCl4)in rats.Methods Rats hepatic fibrosis was induced by CCl4 twice a week for 12 weeks.Since the 7th week,GL was given to model rats daily by intragastric administration for 6 weeks.Contents of malondialdehyde(MDA),superoxide dismutase(SOD),nitric oxide synthase(NOS)and iNOS were detected by absorption spectrometry.The serum TIMP-1 level was examined by enzyme-linked immunospecific assay(ELISA).Hepatic tissue in certain position was sampled,and TIMP-1 expression in the hepatic tissue was detected by immunohistochemical method.Moreover,TIMP-1mRNA expression in liver tissue was detected by RT-PCR technology.Results GG could significantly decrease MDA,NOS,iNOS and TIMP-1 contents,increase SOD level,and inhibit the protein and mRNA expression of TIMP-1 in hepatic tissue of hepatic fibrosis rats.Conclusion GL has an obvious anti-hepatic fibrosis action,and effectively inhibiting the expression of TIMP-1 may be one of its therapeutic mechanisms.