The Effects of DHEA on the PASMC Proliferation Induced by the Hypoxic Endothelial Cells Conditioned Medium

Objective:To investigate the effect of DHEA on the pulmonary arterial smooth muscle cells(PASMC)proliferation stimulated by the hypoxic endothelial cell conditioned medium(HECCM).Methods:the porcine pulmonary arterial endothelial cells(PAEC)and porcine PASMC were cultured in vitro.The norˉmoxic endothelial cell conditioned medium(NECCM)and hypoxic endothelial cell conditioned medium(HECCM)were prepared.The NECCM,HECCM,NECCM+DHEA,HECCM+DHEA were added into the cultured PASMCs respectively.The samples were studied by flow cytometic analysis and PCNA immunohistoˉchemistry on PASMC.Results:Compared with the NECCM group,HECCM could stimulate PASMC prolifˉeration,by promoting the cells from G 0 /G 1 phase into S phase,and enhance the PCNA expression.DHEA could inhibit cultured PASMC proliferation stimulated by the HECCM,and arrested them at the G 0 /G 1 phase,accompanied with remarkable reduction of PCNA expression.Conclusion:The DHEA can inhibit the PASMC proliferation induced by HECCM.