Changes of Glutamine Synthetase Expression in Rat DRG aider Sciatic Nerve Cut

Objective:To observe the changes of glutamine synthetase(GS)expression in dorsal root ganglion(DRG)at different time after sciatic nerve cut.Methods:48 rats were divided randomly into experiment group and normal control group.In the experiment group,right sciatic nerve was operation side and left sciatic nerve was control side.After sciatic nerve cut,rats survived for 1,3,7,14 and 21 day.We employ immunohistochemistry method to detect GS expression change.Results:GS mainly expressed at satellite cell of DRG in control group.GS expression was increasing at 1 day after sciatic nerve cut and apparently higher than that in the control group(P0.05).Then,GS expression was decreasing at 3 day and recovering normal at 7 day.GS expression continued to decrease at 14 day and 21 day,and was lower than that of the control group apparently(P0.05).There was no significant difference about GS expression between the operation side and the control side among experiment group(P0.05).Conclusion:There was space-time changes of GS expression after sciatic nerve cut in DRG,which may be related with excitability toxicity mediated by glumatic acid after sciatic nerve cut in DRG.