Induction of Apoptosis by Epigallocatechin-3-gallate via Activating Mitochondrial Signaling in Human Gastric Cancer Cells

Objective To elucidate the mechanism of apoptosis induced by epigallocatechin-3-gallate(EGCG) on human gastric cancer BGC823 cells via mitochondrial pathway.Methods The survival rate of BGC823 cells was detected by MTT assay.Cell apoptosis was determined by flow cytometry(FCM) using PI staining.Mitochondrial membrane potential was labeled by Rhodamine123 and examined by FCM.Western blot was used to analyze the expression of apoptosis mitochondrial signal transduction pathway related proteins.Results EGCG inhibited proliferation of BGC823 cells in a time-and dose-dependent manner.The apoptosis of BGC823 induced by EGCG was in a dose-dependent manner detected by FCM using PI staining.After treatment with 20 μg/mL,40 μg/mL,80 μg/mL of EGCG,Δψm was decreased,the releasing of cytochrome c was enhanced,the expression of caspase-9 protein was increased in a dose-dependent manner.Conclusion EGCG induces apoptosis of BGC823 cells via activating mitochondrial signal transduction pathway.