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Regulatory Effect of Triptolide on Cell Cycle of Colorectal Cancer Cells

Peng Kang

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Abstract

Objective: To observe the effects of triptolide on the proliferation,cell cycle and apoptosis of colorectal cancer cell line SW480.Methods: SW480 cells were cultured amd treated with triptolide at different concentrations.Inhibition rates of cell growth were detected with MTT assay.The changes of cell cycle and apoptosis rate were detected with flow cytometry(FCM).Rerults: MTT assay showed that triptolide in the range of 20~100 μmol/L significantly inhibited the proliferation of SW480 cells.FCM showed that no significant difference was found in apoptosis rate before and after treatment,but S phase cells increased significantly and cell cycle was obviously blocked.Conclusions: Triptolide can inhibit SW480 cell proliferation in a dose and time dependent manner,which may be a result of cell cycle arrest.

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What this paper is about

Objective: To observe the effects of triptolide on the proliferation,cell cycle and apoptosis of colorectal cancer cell line SW480.Methods: SW480 cells were cultured amd treated with triptolide at different concentrations.Inhibition rates of cell growth were detected with MTT assay.The changes of cell cycle and apoptosis rate were detected with flow cytometry(FCM).Rerults: MTT assay showed that triptolide in the range of 20~100 μmol/L significantly inhibited the proliferation of SW480 cells.FCM showed that no significant difference was found in apoptosis rate before and after treatment,but S phase cells increased significantly and cell cycle was obviously blocked.Conclusions: Triptolide can inhibit SW480 cell proliferation in a dose and time dependent manner,which may be a result of cell cycle arrest.

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Available abstract

Objective: To observe the effects of triptolide on the proliferation,cell cycle and apoptosis of colorectal cancer cell line SW480.Methods: SW480 cells were cultured amd treated with triptolide at different concentrations.Inhibition rates of cell growth were detected with MTT assay.The changes of cell cycle and apoptosis rate were detected with flow cytometry(FCM).Rerults: MTT assay showed that triptolide in the range of 20~100 μmol/L significantly inhibited the proliferation of SW480 cells.FCM showed that no significant difference was found in apoptosis rate before and after treatment,but S phase cells increased significantly and cell cycle was obviously blocked.Conclusions: Triptolide can inhibit SW480 cell proliferation in a dose and time dependent manner,which may be a result of cell cycle arrest.

Key concepts: Triptolide, Cell cycle, Apoptosis, Flow cytometry, Cell growth, MTT assay, Cell, Cell cycle checkpoint

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Regulatory Effect of Triptolide on Cell Cycle of Colorectal Cancer Cells — Research Paper | ScholarLens