Simultaneous determination of nine constituents in the roots and rhizomes of Glycyrrhiza uralensis from different producing areas by RP-HPLC

Objective:To develop an HPLC method for simultaneous determination of 9 main constituents(liquiritin apioside,liquiritin,isoliquiritin apioside,isoliquiritin,licochalcone B,liquiritigenin,echinatin,isoliquiritigenin,glycyrrhizic acid)in the roots and rhizomes of Glycyrrhiza uralensis Fisch.Methods:The analyses were performed on a DiamonsilTM ODS C18 column(250 mm × 4.6 mm,5 μm)with the gradient elution of acetonitrile-0.05% phosphoric acid aqueous at a flow rate of 0.8 mL·min-1.The detection wavelength was set at 276 nm(0-20 min),360 nm(20-30 min),276 nm(30-35 min),370 nm(35-48 min),248 nm(48-55 min)and the column temperature was at 40 °C.Results: Good linearity was obtained for liquiritin apioside,liquiritin,isoliquiritin apioside,isoliquiritin,licochalcone B,liquiritigenin,echinatin,isoliquiritigenin,and glycyrrhizic acid in ranges of 13.1-262 μg·mL-1(r=0.9994),8.3-100 μg·mL-1(r=0.9997),5.2-104 μg·mL-1(r=0.9997),0.96-11.5 μg·mL-1(r=0.9995),0.31-10.00 μg·mL-1(r=0.9999),1.11-22.2 μg·mL-1(r=0.9991),0.23-7.25 μg·mL-1(r=0.9998),0.52-10.40 μg·mL-1(r=0.9996)and 25.25-808.0 μg·mL-1(r=0.9995),respectively.The average recovery(n=6)of the nine constitutions was 103.6%、104.5%、104.9%、100.4%、102.2%、101.5%、100.4%、98.5%、101.7%,respectively.Conclusion:The developed HPLC method is simple and repeatable,which is helpful for the quality control of the roots and rhizomes of G.uralensis.