The Establishment of Indirect ELISA Assay for the Major Antigenic Domain of Porcine parvovirus VP2 protein

Followed the transformation of vp2 gene of PPV into the competent cell of Pichia pastoris,the vp2 protein of PPV was successfully expressed under the optimal expression conditions.The final concentration recombinant Vp2 protein was 227.6μg/ml,using the recombinant protein,an indirect ELISA assay for detection of PPV antibody was established.The optimal working circumstances for the antigen conditions(5.69μg/ml) and the serum dilution were 1∶80,determined chess titration.The positive circumstances of this ELISA assay is OD490 value of the sample serum 0.5,and the ratio of the tested serum OD490 to the negative serum is higher than 2.0.This ivp2-ELISA assay was compared with HA/HI and PPV ELISA kit of Cedi company,result shows the agreement ratio is 97.2% and 91.2%.