Expression of Nestin Protein and Its Relation with Vascular Endothelia Growth Factor in Brain of Neonatal Rats with Hypoxia-Ischemia Brain Damage

Objective To explore the relationship between the expression of nestin protein and the expression of vascular endothelia growth factor(VEGF)in hypoxia-ischemia brain damage(HIBD)of neonatal SD rats.Methods Fifty-six 7-day postnatal SD rats were randomly divided into 2 groups,sham operation(n=28)and hypoxic ischemic(HI)group(n=28).Animals were killed in 0,3,12,24 h,3,7,14 d after HIBD rat models established.The brain tissue was obtained to make paraffin section.Expressions of nestin and VEGF protein were examined with immunohistochemical staining and image quantitative analysis.Results The number of VEGF positive cell in the hippocampus and cortex increased reactively after HI and reached peak at the 12th hour and persistent to the 24th hour,which returned the normal level on d3,however,the number of nestin positive cell reached peak on d7 and returned the normal level on d14 after HI.Conclusions VEGF and nestin may contribute to neurolproctetive effects against HIBD.Studying the way to facilitate the coexpression of the nestin and VEGF may have important rolls to enhance the repairment and regeneration.