Antifungal metabolites of endophytic fungus of 2QR1 strain in Euonymus japonicus

A endophytic fungi strain was isolated from Everyreen euonymus.The strain numbered 2QR1 was preliminarily identified as deuteromycotina Aspergillum.The result of fungicidal activities of metabolities of 2QR1 strain showed that the EC50 value of ethyl acetate extract of fermentation broth of 2QR1 strain was separately 53.6,63.1,63.3,98.2,106.5,115.9 and 166.3 μg/mL against Bipolaris sorokiniana,Fusarium graminearum,Glomerella cingulata,Exserohilum turcicum,Fulvia fulvum,Alternaria longipe and Fusarium solani.The EC50 value of acetate extract of fermentation broth of 2QR1 to inhibit the spore germinations of Exserohilum turcicum,Alternaria longipes,Bipolaris sorokiniana and Fusarium solani was separately 94.8,139.1,146.6 and 180.1 μg/mL.The inhabition zone diameter of ethyl acetate extract of fermentation broth of 2QR1 strain was more than 16mm at the concentration of 500 μg/mL against Bacillus subtilis,Bacillus cereus and Staphylococcu aureus.The results of pot test indicated that ethyl acetate extract of fermentation broth of 2QR1 strain showed 61.9% of protected efficacy and 81.6% of therapeutic efficacy at 2 000 μg/mL.Compound B9 was separated from the ethyl acetate extract of fermentation broth by p-TLC.The result of spore germination proved that the EC50 value of compound B9 was separately 0.8,1.4,3.3和5.0 μg/mL,against Exserohilum turcicum,Alternaria longipes,Bipolaris sorokiniana and Fusarium solani.