THE EFFECT OF CURCUMIN ON NON-ANDROGEN-DEPENDENT PC-3 CELL APOPTOSIS OF PROSTATE CANCER

Objective To observe the effect of curcumin on proliferation and apoptosis of PC-3 cells in non-androgen-dependent prostate cancer. Methods Different concentrations of curcumin-0,10,20,30 and 40 μmol/L-were used to act on PC-3 cells for 48 h,the survival rate of the cells was then determined using Cell Counting Kit-8(CCK8);0,5,10,and 20 μmol/L curcumin were used to act on PC-3 cells for 48 h and then the cell morphology was observed by Hoechst staining.Annexin V/PI was applied for apoptosis detection. Results Curcumin could significantly inhibit the PC-3 cell proliferation(F=36.82,q=4.88-15.36,P0.01).The differences of the cell apoptosis rates in different-concentration groups were significant,except for the 0 and 5 μmol/L groups(F=7.88,q=4.15-6.54,P0.01).After acting with 20 μmol/L curcumin for 48 h,the morphological changes such as chromatic agglutination,karyopyknosis,and nuclear fragmentation could be seen in part of apoptotic cells with inverted microscope. Conclusion Curcumin could,in vitro,dramatically inhibit PC-3 cell proliferation and induce apoptosis,which provides an experimental evidence for therapy of non-hormone-dependent prostatic cancer.