Expressions of STAT3 gene in human hepatocellular carcinoma cell lines HepG2,Bel7402,SMMC7721 and hepatocellular carcinoma tissues and their significances

Objective To explore the role and possible mechanism of abnormal activation of STAT3 gene during the occurrence and development of human hepatocellular carcinomar(HCC).Methods Immunohistochemistry dyeing,RT-PCR and Western blotting were used to detect the mRNA and protein expressions of STAT3 gene in HCC cell lines SMMC7721,Bel7402 and HepG2 and human HCC tissues.Results There were high expressions of STAT3 gene in SMMC7721,Bel7402 and HepG2 cells,and there was no significant difference among them(P0.05).There were high expressions of STAT3 gene at the mRNA and protein levels in HCC tissues and tissues surrounding carcinoma,and there was significant difference between normal tissues and HCC tissues(P0.05),while there was no difference between HCC tissues and tissues surrounding carcinoma(P0.05).The mRNA and protein expressions of VEGF,survivin and c-myc genes were increased in HCC tissues,while the mRNA and protein expressions of p53 were decreased in HCC tissues.Conclusion The persistent activation of STAT3 gene may occur at early stage of HCC pathogenesis and plays an important promoting role in the carcinogenesis of HCC.