Effect of juglone at different concentrations on HeLa cells

Objective To detect the effect of juglone at different concentrations on growth and proliferation of HeLa cells.Methods The human HeLa cell line was cultivated in vitro.The changes of morphology were observed by inverted microscope;the propagation was detected by MTT and the cell cycle and cell apoptosis were detected by flow cytometer.Results Cytomorphology trial showed that juglone at different concentrations could lead to changes of cellular morphology.MTT showed that there was significant difference in juglone at different concentrations compared with blank control group(P0.05,P0.01);the flow cytometer showed that after 24 hours,the G2/M phase was appeared in HeLa cells and the apoptosis rate was increased.Compared with control group,there was significant difference in juglone at different concentrations(P0.05,P0.01);the Caspase-3 activity assay showed that juglone at different concentrations could increase the activity of Caspase-3,and which was significantly different from the control group(P0.05,P0.01);all the results above had dose-dependent.Conclusion Juglone at different concentrations can inhibit the proliferation of HeLa cells,which have dose-dependent,and expected to become the new anti-tumor therapeutic targets.