Improving of experiment on PCR rapid screening for recombinant clones

Analysing the problems on PCR Screening for vecombinant clone,we put forward suggesttions to them:The aimed cDNA fragments were acquired by RT-PCR.The products were inserted to the cloning T-vectors.DH5α(E.coli) transformed by recombinant plasmid was inoculated.The same size positive strips as the cDNA fragments were visible in Bacteria Liquid PCR of the screened positive clones with specific primers for amplificating target genes.The results were in great agreement with those obtained by plasmid PCR and double enzyme digestion as positive controls.The Conclusion is that Bacteria Liquid PCR is a simple,efficient and reliable technique for screening positive clones of recombinant genes.