Residue analysis of flubendiamide and its metabolite in rice by UPLC

A UPLC method of flubendiamide and its metabolite for determination in water,soil,straw,brown rice and rice hull was established.Water samples were purified by extractant ethyl acetate through liquid-liquid extraction.Soil samples were extracted with acetone and purified as water samples.Rice samples were extracted with acetonitrile,and cleaned up by NH2-Carb columns.The average recoveries were 78.2%—104.8%,with variation coefficients of 1.1%—4.4%.The minimum detectable quantity of flubendiamide and its metabolite were 0.004—0.02 ng,and the limits of detection ranged from 0.0008 to 0.0009 mg · L-1 in water,and from 0.001 to 0.003 mg · kg-1 in soil,straw,brown rice and rice hull.