AN IMMUNOHISTOCHEMICAL AND FOLLOW-UP STUDY OF 148 INTRACRANIAL TUMORS

Objective:To study the value of glial fibrillazy acidic protein(GFAP) and S-100 protein immunohistochemical staining to the diagnosis of the intracranial tumors.Methods:Resected tumor samples of 148 intracranial tumors were fixed in formalin and embedded in paraffin. The 5μm tumor sections were used for histology (hematoxylin and eosin stain),and consecutive sections were stained immunohistocchemically with GFAP and S-100 protein antibodies.55 gliomas were followed up.Result:GFAP was present in different varieties of astrocytomas,ependymoma,multiforme glioblastoma,eligodendroglioma,astrocytic elements of mixed gliomas.S-100 protein existed in both nonglial tumors and glial origin tumors.With the increase in malignancy of the glioma,the positive staining Yate of GFAP was decreased (P0.001), the positive staining sate of GFAP was decreased (P0.001),two-years survival rate was lower and the prognose was poorer (P0.001).Conclusion:GFAP and S-100 immunohistochemical stainings are helpful to the diagnosis and differentiated diagnosis of incranial tumors.Detection of GFAP expression may have significant value in the accuracy of glial origin tumor and evaluation of the prognosis. [