Optimization of Separation and Purification Technology for Total Flavonoids and Puerarin from Pueraria lobata by Macroporous Adsorption Resin

Objective:To optimize separation and purification technology of total flavonoids and puerarin from Pueraria lobata by macroporous adsorption resin.Method:Static adsorption and desorption performance of four kinds of different polar macroporous adsorption resins(S-8,AB-8,D-101,HPD-100) for total flavonoids and puerarin from P.lobata were investigated,in order to select optimal resin;With yield and purity of total flavonoids and puerarin as indexes,single factor test was used to investigate adsorption and elution conditions.Result:AB-8 macroporous resin was chosen,its optimized technology conditions were as followings:the concentration of sample solution 1.0 g·mL-1,mass ratio of raw material-resin 1∶2,diameter-height ratio of column 1∶6,washed by 2 BV water,eluted with 2 BV 80% ethanol,elution flow rate 2 mL·min-1.Elution rate of total flavonoids and puerarin were more than 92%,purity of total flavonoids and puerarin could reach 80% and 25%,respectively.Conclusion:AB-8 macroporous adsorption resin was sutiable for enrichment total flavonoids and puerarin from P.lobata,it was a kind of ideal separation and purification medium,optimized technology was simple and reliable.