Experimental Study on Activation of Caspase-3 and Apoptosis of K562 Cell Induced by Iron-Deprivation

Objective To observe the relationship between apoptosis of K562 cell induced by iron-deprivation and activation of Caspase-3.Methods K562 cells were treated with desferrioxamine(DFO) in different dosages were collected at different time points.K562 cells were labelled with Annexin V/PI,and then the rate of apoptosis was measured by flow cytometry;The activation of Caspase-3 were detected by colorimetric method with pAN labelled substrate;The active protein of Caspase-3 were analyzed by Western blot.Results When K562 cells were treated with different concentrations of DFO,the apoptosis rate and the activity of Caspase-3 increases gradually.When K562 cells were incubated with DFO(50 μmol/L and 100 μmol/L) 24 h later,the enzymatic activity of Caspase-3 increases dramatically more than that of control group,and the difference was significantly(P0.001);The active protein of Caspase-3 could been found when K562 cells were treated with DFO(100 μmol/L) 14 h later,the activity and the quantity of Caspase-3 depends on time-dosage;Compared with control group at 10 h and 12 h time point,the activation of Caspase-3 was not different significantly from each other(P0.05).All those effect above can be counteracted by equal mole concentration of FeCl_3.Conclusion Iron-deprivation maybe induce the apoptosis of K562 cell by chelating intracellular iron and activing Caspase-3.