Inhibitory Effect of Ginsenoside Rg3 on Tumor Neoangiogenesis in Transplanted Human Hepatic Carcinoma

Objective To study the effect of reducing the intratumoral microvessel density(MVD) by Ginsenoside Rg3(Rg3) in transplanted human hepatic carcinoma in nude mice. Methods 24 male nude mice were randomly divided into 4 groups to receive Rg3(10 mg/kg) combined with Arsennic trioxide(As_2O_3),Rg3(10 mg/kg) alone,Arsennic trioxide(As_2O_3)(40μg/kg·d) alone and Saline(0.5 ml,qd) respectively.3 days after being inoculated in nude mice,Rg3 was given by gastrogavage once every 2 days,for total 20 times,As_2O_3 was injected introperitoneally once a day for 28 days,a week after treatment being stopped,mice were killed and the sizes of solid tumors were measured,the intratumor MVD was examined by immunohistochemical staining. Results The tumor weight of treated group was significantly lower than that of control group.The tumor weight of Rg3 combined with As_2O_3 group was lower than that of Rg3 group.The MVD value of Rg3 group was significantly lower than that of As_2O_3 group and control group. Conclusion Rg3 can obviously reduce the intratumoral MVD through inhibiting aniogensis of malignant tumor.Rg3 combined with As_2O_3 can significantly inhibit the growth of transplanted human hepatic carcinoma in nude mice.