Establishment of Human Immune Reconstitute NOD/SCID Mice Model

Objective To study the establishment method of human immune reconstitute animal model in NOD/SCID mice and the characteristics of immunologic reconstitution.Methods 16 NOD/SCID mice were randomly divided into experimental group(n=8) and control group(n=8).The peripheral blood mononuclear cells(PBMCs)were isolated from fresh peripheral blood of healthy people using Ficoll lymphocyte separating medium,and then were transplanted into experimental mice by intraperitoneal injection.Mice in control group was injected PBS buffer.In the 4th,8th and 12th weeks,human CD3 T and CD19 B lymphocytes in the blood of mice were detected by flow cytometry,human IgG protein content in the blood of mice was measured by ELISA method and the infiltration of human CD3 T and CD19 B lymphocytes in mice spleen and liver were detected by immunohistochemical staining.Results After 4 weeks,human CD3 T and CD19 B lymphocytes in peripheral blood of experimental mice were respectively 85.6% and 76.7% of the total monocytes,and were also detected in mice spleen.The amount of human IgG in serum of experimental mice after 4,8 and 12 weeks of transplantation were respectively(863±12.5) μg/mL,(1217±16.7) μg/mL and(958±13.1) μg/mL.Conclusions Human immune reconstitute NOD/SCID mice model can be successfully established by intraperitoneal injection of human PBMC.The method is simple and reliable.